Plant Essential Oils as Healthy Functional Ingredients of Nutraceuticals and Diet Supplements: A Review

Essential oils (EOs) are mixtures of volatile molecules endowed with health-promoting biological activities that go beyond their role as aromas and natural preservatives and can be exploited to develop functional foods and diet supplements. Their composition is briefly addressed along with regulatory aspects. The potential health benefit of human diet supplementation with EOs is outlined through a review of the recent literature on available clinical trials and preclinical research concerning EOs activity towards: (1) irritable bowel syndrome; (2) inflammatory bowel disease; (3) regulation of microbiota; (4) gastroprotection; (5) hepatoprotection; (6) protection of the urinary tract and diuresis; (7) management of metabolic disorders including hyperglycemia and hyperlipidemia; (8) anti-inflammatory and pain control; (9) immunomodulation and protection from influenza; and (10) neuroprotection and modulation of mood and cognitive performance. The emerging potential in such activities of selected EOs is given focus, particularly green and black cumin, bergamot, orange, myrtle, peppermint, sage, eucalyptus, lavender, thyme, lemon balm, ginger, and garlic

Decreasing time consumption of microscopy image segmentation through parallel processing on the GPU

The computational performance of graphical processing units (GPUs) has improved significantly. Achieving speedup factors of more than 50x compared to single-threaded CPU execution are not uncommon due to parallel processing. This makes their use for high throughput microscopy image analysis very appealing. Unfortunately, GPU programming is not straightforward and requires a lot of programming skills and effort. Additionally, the attainable speedup factor is hard to predict, since it depends on the type of algorithm, input data and the way in which the algorithm is implemented. In this paper, we identify the characteristic algorithm and data-dependent properties that significantly relate to the achievable GPU speedup. We find that the overall GPU speedup depends on three major factors: (1) the coarse-grained parallelism of the algorithm, (2) the size of the data and (3) the computation/memory transfer ratio. This is illustrated on two types of well-known segmentation methods that are extensively used in microscopy image analysis: SLIC superpixels and high-level geometric active contours. In particular, we find that our used geometric active contour segmentation algorithm is very suitable for parallel processing, resulting in acceleration factors of 50x for 0.1 megapixel images and 100x for 10 megapixel images

Frequent activity on Vulcano (Italy) spanning the last 80 ky: New insights from the chemo-stratigraphy of the Brown Tuffs

The Brown Tuffs (BT) are widespread reddish-brown to grey, ash-rich pyroclastic deposits recognized in the stratigraphic sequences of the Aeolian Islands and Capo Millazzo peninsula (Sicily) that span the last 80 ky. They have very homogeneous lithological, textural and sedimentological features which make it difficult to reliably correlate units on the islands to proximal units in the source areas. Here we carefully re-interpret the stratigraphic profiles of the BT on Vulcano and Lipari where the deposits are thickest and present the most complete succession. The investigation is based on a large dataset of major and minor element geochemistry of juvenile glass components for the majority of the recognized BT depositional units, whilst also providing new radiocarbon ages. The distinctive chemical groupings observed within the glass analyses, both temporally and spatially, allow us to fingerprint the three main stratigraphically defined macro-units in which the BT succession can be sub-divided using prominent tephra marker beds, the Ischia Tephra (Monte Epomeo Green Tuff; 56 ky) and Monte Guardia pyroclastics from Lipari (herein radiocarbon dated to 27\u201326 ky). The Lower (80\u201356 ky; LBT), Intermediate (56\u201327 ky; IBT) and Upper BT (here dated at 24\u20136 ky; UBT) macro-units display K-series volcanic glasses ranging from basaltic trachy-andesites, through trachy-andesites, to more evolved trachytes, all consistent with an origin on Vulcano. The UBT are clearly distinguished from the lower macro units by their higher-SiO2 trachy-andesite to trachytic glasses, which extend to noticeably lower TiO2, CaO and MgO contents. These features make it possible to re-define the geochemical-evolutionary boundary between IBT and UBT as corresponding to the 24 ky Spiaggia Lunga scoria bed on Vulcano, which is stratigraphically higher (and younger) than the previous boundary marker (Monte Guardia). The glass compositions of the LBT, IBT and UBT are used to: (1) assess links to known proximal eruption units outcropping on Vulcano; (2) validate medial-distal BT occurrences across the Aeolian archipelago (Salina, Filicudi and Panarea) and on Capo Millazzo; (3) confirm that the BT are responsible for distal volcanic ash layers preserved in Central Mediterranean marine sedimentary archives. Interestingly, the glass compositions of the UBT are very similar to those of the Punte Nere unit, the earliest pyroclastic products erupted from the currently active La Fossa cone on Vulcano, indicating the corresponding magmatic system has likely erupted similar melts and products over the last 24 ky and thus extending its life cycle. Such information is crucial for evaluating the long-term eruption scenarios underpinning hazard assessment of the La Fossa caldera magmatic system

Design and construction of a new metering hot box for the in situ hygrothermal measurement in dynamic conditions of historic masonries

The main purpose of the HeLLo project is to contribute to data available on the literature on the real hygrothermal behavior of historic walls and the suitability of insulation technologies. Furthermore, it also aims at minimizing the energy simulation errors at the design phase and at improving their conservation features. In this framework, one of the preliminary activities of the study is the creation of a real in situ hot box to measure and analyze different insulation technologies applied to a real historic wall, to quantify the hygrothermal performance of a masonry building. Inside this box, 'traditional' experiments can be carried out: recording heat flux, surface temperature, and air temperatures, as well as relative humidity values through the use of a new sensing system (composed of thermocouples and temperature/relative humidity combined sensors). Within this paper, the process of development, construction, and validation of this new metering box is exhibited. The new hot box, specifically studied for historic case studies, when compared to other boxes, presents other advantages compared to previous examples, widely exemplified

Electrographic changes accompanying recurrent seizures under ketogenic diet treatment.

The ketogenic diet (KD) is increasingly used to treat epilepsy refractory to antiepileptic drugs and other neurological disorders. In animal models, the KD was found to increase the threshold to seizures induced by different convulsive stimulations. However, in models in which suprathreshold stimuli were used, a paradoxical seizure worsening was consistently observed in KD-fed animals. To better define this phenomenon, we characterized the electrographic response to seizures induced in mice which were treated with the KD, and then corneally stimulated at 6-Hz in four different sessions. We also evaluated the electroencephalogram (EEG) in three patients in which the KD was associated with a paradoxical worsening of epileptic seizures. Although seizures were initially less severe, a remarkable prolongation of the electrographic response was observed in mice receiving the KD from the second session of 6-Hz corneal stimulation and onwards. The EEG was also markedly altered in the presence of progressive seizure aggravation observed in children treated with the KD, specifically one affected by Lennox\u2013Gastaut syndrome and two by type I lissencephaly. These results suggest that when seizures are induced or recur because of resistance to therapeutic interventions, the KD may change the EEG by potentiating the electrographic epileptic activity

Development of a compatible, low cost and high accurate conservation remote sensing technology for the hygrothermal assessment of historic walls

This article aims to properly assess the hygrothermal properties of walls located in historic buildings, this study discloses the development of a remote sensing technology compatible with an in-situ measurement implemented in Palazzo Tassoni (Italy). As required by the international recommendations adapted to cultural heritage (CH), this monitoring system balances CH conservation, performance aspects and economic costs using an integrated multidisciplinary approach. Electronics for measurement of environmental parameters is composed of sensor measurements, data acquisition system and data storage and communication system. Data acquisition system, equipped with standard modbus-rtu interface, is designed to run standalone and it is based on two cloned single board PCs to reduce the possibility of data loss. In order to reduce the costs, RaspberryPI single board PCs were chosen. These run a C/C++ software based on standard modbus library and designed to implement multi-client server TCP/IP to allow communication with other devices. Storage and communication systems are based on an industrial PC; it communicates with sensor measurements' system through a modbus-TCPIP bridge. PC runs a Labview software to provide data storage on a local database and graphical user interface to properly see all acquired data. Herein, some sensing options and approaches of measurement are described, unveiling different possible ways of enhancing the retrofit of CH with adapted technology

Exploiting Enclosing Membranes and Contextual Cues for Mitochondria Segmentation

In this paper, we improve upon earlier approaches to segmenting mitochondria in Electron Microscopy images by explicitly modeling the double membrane that encloses mitochondria, as well as using features that capture context over an extended neighborhood. We demonstrate that this results in both improved classification accuracy and reduced computational requirements for training

Malignant pleural mesothelioma and mesothelial hyperplasia: A new molecular tool for the differential diagnosis

Malignant pleural mesothelioma (MPM) is a rare asbestos related cancer, aggressive and unresponsive to therapies. Histological examination of pleural lesions is the gold standard of MPM diagnosis, although it is sometimes hard to discriminate the epithelioid type of MPM from benign mesothelial hyperplasia (MH). This work aims to define a new molecular tool for the differential diagnosis of MPM, using the expression profile of 117 genes deregulated in this tumour. The gene expression analysis was performed by nanoString System on tumour tissues from 36 epithelioid MPM and 17 MH patients, and on 14 mesothelial pleural samples analysed in a blind way. Data analysis included raw nanoString data normalization, unsupervised cluster analysis by Pearson correlation, non-parametric Mann Whitney U-test and molecular classification by the Uncorrelated Shrunken Centroid (USC) Algorithm. The Mann-Whitney U-test found 35 genes upregulated and 31 downregulated in MPM. The unsupervised cluster analysis revealed two clusters, one composed only of MPM and one only of MH samples, thus revealing class-specific gene profiles. The Uncorrelated Shrunken Centroid algorithm identified two classifiers, one including 22 genes and the other 40 genes, able to properly classify all the samples as benign or malignant using gene expression data; both classifiers were also able to correctly determine, in a blind analysis, the diagnostic categories of all the 14 unknown samples. In conclusion we delineated a diagnostic tool combining molecular data (gene expression) and computational analysis (USC algorithm), which can be applied in the clinical practice for the differential diagnosis of MPM

Clastic vs. primary precipitated evaporites in the Messinian sicilian Basins

The Messinian stratigraphy of Sicily has a particular importance for the comprehension ofthe Messinian salinity crisis as its successions bear the greatest similarity with those of thedeep Mediterranean basins. Despite the large number of studies carried out in the last 30years, we believe that the true time and genetic relationships between the different evaporiticand non evaporitic rock bodies are still not well established. This is probably due to thelimited, partial view offered by the central Sicilian basin, despite its complete Messinianstratigraphic record.Clastic and chaotic evaporitic deposits emplaced by tectonically-driven small to largescaleresedimentation processes form an important part of the MSC record of Sicily in theBelice and Caltanissetta basins. Facies characteristics of clastic evaporites, the stratigraphicrelationships with the other Messinian deposits, their possible significance in the regionalgeological evolution and the implications at a Mediterranean scale will be discussed in thefield. Attention also will be paid to primary precipitated facies of Lower and UpperEvaporites.The main aim of this field trip is to visit and discuss, beside some of the classic localitiesof the Caltanissetta basin, other less known outcrops of western Sicily (Belice basin), inorder to have a more complete regional geological framework of the MSC events in Sicily.This will give the participants the opportunity to discuss many of the still open problemsconcerning the MSC. In this section we suggest some topics for discussion during the fieldtrip

Proposal of A New Bois Noir Epidemiological Pattern Related to ‘Candidatus Phytoplasma Solani’ Strains Characterized by A Possible Moderate Virulence in Tuscany

Bois noir (BN), associated with 'Candidatus Phytoplasma solani' (CaPsol), is the most widespread disease of the grapevine yellows complex worldwide. In this work, BN epidemiology was investigated in a case study vineyard where an unusual CaPsol strain, previously detected only in other host plants, was found to be prevalent in grapevine. Experimental activities included: symptom observation; sampling of symptomatic vines, Auchenorrhyncha specimens, and weeds; molecular detection and typing of CaPsol strains; statistical analyses for determining possible relationships between CaPsol relative concentration, strain type, and symptom severity. Among insects, Reptalus quinquecostatus was the most abundant and was found to be highly infected by CaPsol, while Hyalesthes obsoletus, the main CaPsol vector, was not caught. Moreover, R. quinquecostatus harbored CaPsol strains carrying uniquely the stamp sequence variant St10, also identified as prevalent in vines and in the majority of weeds, and all the secY variants identified in the vineyard. Statistical analyses revealed that CaPsol strains carrying the St10 variant are not associated with severe symptoms, suggesting their possible moderate virulence. Based on such evidence, a new BN epidemiological pattern related to these CaPsol strains and involving grapevine, R. quinquecostatus, and/or weeds is proposed. Furthermore, the possible presence of other players (vectors and weeds) involved in CaPsol transmission to grapevines was highlighted